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HighYield T7 RNA Synthesis Kit
| 貨號 | RNT-101 | 售價(jià)（元） | 2925 |
| 規格 | 50T x 20 μl | CAS號 |
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產(chǎn)品信息
|
品牌 |
貨號 |
名稱(chēng) |
規格 |
單價(jià) |
|
Jena Bioscience |
50T x 20 μl |
2925.00 |
產(chǎn)品簡(jiǎn)介
HighYield T7 RNA合成試劑盒旨在通過(guò)T7 RNA聚合酶的體外轉錄產(chǎn)生大量RNA。它也適用于加入修飾核苷酸（例如生物素或熒光標記的NTP或cap類(lèi)似物）。
由此產(chǎn)生的RNA隨后可用于多種應用，如微注射、轉染或體外翻譯實(shí)驗、結構/功能研究或原位雜交探針和Northern Blot實(shí)驗。
該試劑盒包含足夠的試劑，用于50個(gè)反應，每個(gè)反應20μl（每個(gè)NTP 7.5 mM）。培養30分鐘后，20μl反應產(chǎn)生約140-160μg RNA（1μg T7對照模板，1.4 kb RNA轉錄物）。然而，產(chǎn)量可能因模板而異（啟動(dòng)子設計、序列長(cháng)度、二級結構形成）。
Content:
HighYield T7 RNA Polymerase Mix
3x 40 μl incl. RNase inhibitor and 50 % glycerol (v/v)
HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based
ATP - Solution
1x 100 μl (100 mM)
GTP - Solution
1x 100 μl (100 mM)
CTP - Solution
1x 100 μl (100 mM)
UTP - Solution
1x 100 μl (100 mM)
T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor
resulting in ~1400 nt RNA transcript
T7 A-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class II phi2.5 promotor
(A-initiating) resulting in ~1400 nt RNA transcript
PCR-grade water
1x 1.2 ml
DTT
1x 100 μl (100 mM)
To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I
|
品牌 |
貨號 |
名稱(chēng) |
規格 |
單價(jià) |
|
Jena Bioscience |
RNT-106-S |
HighYield T7 mRNA Synthesis Kit (Ψ-UTP) |
15reactions x 20 μl |
4425.00 |
|
Jena Bioscience |
RNT-106-L |
HighYield T7 mRNA Synthesis Kit (Ψ-UTP) |
50reactions x 20 μl |
8925.00 |
|
Jena Bioscience |
RNT-105 |
HighYield T7 sgRNA Synthesis Kit (SpCas9) |
50reactions |
4425.00 |
|
Jena Bioscience |
RNT-104 |
HighYield T7 mRNA Synthesis Kit (m5CTP/Ψ-UTP) |
15reactions x 20 μl |
7875.00 |
|
Jena Bioscience |
RNT-103 |
HighYield T7 ARCA mRNA Synthesis Kit (m5CTP/Ψ-UTP) |
15reactions x 20 μl |
8805.00 |
|
Jena Bioscience |
RNT-102-S |
HighYield T7 ARCA mRNA Synthesis Kit |
15reactions x 20 μl |
4125.00 |
|
Jena Bioscience |
RNT-102-L |
HighYield T7 ARCA mRNA Synthesis Kit |
50reactions x 20 μl |
8175.00 |
|
Jena Bioscience |
RNT-101-FAMX |
HighYield T7 Fluorescein RNA Labeling Kit |
20reactions x 20 μl |
4875.00 |
|
Jena Bioscience |
RNT-101-DIGX |
HighYield T7 Digoxigenin RNA Labeling Kit |
30reactions x 20 μl |
4425.00 |
|
Jena Bioscience |
RNT-101-CY5 |
HighYield T7 Cy5 RNA Labeling Kit |
20reactions x 20 μl |
4875.00 |
|
Jena Bioscience |
RNT-101-CY3 |
HighYield T7 Cy3 RNA Labeling Kit |
20reactions x 20 μl |
4875.00 |
|
Jena Bioscience |
RNT-101-BIOX |
HighYield T7 Biotin11 RNA Labeling Kit |
30reactions x 20 μl |
4425.00 |
|
Jena Bioscience |
RNT-101-BIO16 |
HighYield T7 Biotin16 RNA Labeling Kit |
30reactions x 20 μl |
4425.00 |
|
Jena Bioscience |
RNT-101-AZ |
HighYield T7 Azide RNA Labeling Kit |
25reactions x 20 μl |
3975.00 |
|
Jena Bioscience |
RNT-101-AF647 |
HighYield T7 AF647 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-AF594 |
HighYield T7 AF594 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-AF555 |
HighYield T7 AF555 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-AF488 |
HighYield T7 AF488 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-488-S |
HighYield T7 Atto488 RNA Labeling Kit |
20reactions x 20 μl |
5925.00 |
|
Jena Bioscience |
RNT-101-488-L |
HighYield T7 Atto488 RNA Labeling Kit |
50reactions x 20 μl |
13755.00 |
除上述mRNA體外合成試劑盒外，Jena Bioscienc還提供一系列相關(guān)高質(zhì)量原料。包括：修飾核苷酸、T7 RNA聚合酶、加帽酶、加尾酶等。
南京沃博生物科技有限公司致力于為廣大科研工作者提供專(zhuān)業(yè)高品質(zhì)實(shí)驗室公用產(chǎn)品，歡迎撥打服務(wù)熱線(xiàn)025-57795736,QQ:1076801270或登錄網(wǎng)站www.www.mycelticfaith.com了解更多信息。