HighYield T7 Biotin11 RNA Labeling Kit_南京沃博生物科技有限公司

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HighYield T7 Biotin11 RNA Labeling Kit

貨號	RNT-101-BIOX	售價(jià)（元）	4425
規格	30Tx 20 μl	CAS號

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產(chǎn)品信息

品牌	貨號	名稱(chēng)	規格	單價(jià)
Jena Bioscience	RNT-101-BIOX	HighYield T7 Biotin11 RNA Labeling Kit	30Tx 20 μl	4425.00

產(chǎn)品簡(jiǎn)介

HighYield T7 Biotin11 RNA標記試劑盒設計用于通過(guò)體外轉錄產(chǎn)生帶有11個(gè)原子連接子的隨機生物素修飾RNA探針。這種探針?lè )浅＿m合原位雜交和Northern Blot實(shí)驗。使用優(yōu)化的反應緩沖液和T7 RNA標記聚合酶混合物，生物素-11-UTP作為天然對應物UTP的替代物有效地并入RNA。35%的生物素-11-UTP替代通常會(huì )在反應和標記效率之間實(shí)現最佳平衡。然而，生物素-11-UTP/UTP比值的個(gè)體優(yōu)化可以通過(guò)單核苷酸格式輕松實(shí)現。由此產(chǎn)生的帶有11個(gè)原子連接子的生物素修飾RNA探針隨后可以被HRP或AP修飾的鏈親和素檢測到。

試劑盒包含足夠的試劑，用于30個(gè)標記反應，每個(gè)反應20μl（35%生物素-11-UTP替代物，1 mM ATP，GTP，CTP，0.65 mM UTP，0.35 mM生物素-11-UTP）。

Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

Biotin-11-UTP
1x 30 μl (10 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)

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