產(chǎn)品信息

      AmpliScribe?T7高產(chǎn)轉錄試劑盒特殊配方使得用戶(hù)能夠從體外轉錄反應中獲得最大可能的RNA產(chǎn)量。利用1μg對照模板在標準的2小時(shí)，20-μL AmpliScribe反應體系中，將摻入反應體系中高達90％的NTP，并產(chǎn)生高達150μg的RNA。

      The AmpliScribe? T7 High Yield Transcription Kit is specially formulated to enable users to obtain the maximum possible yields of RNA from an in vitro transcription reaction. The standard 2-hour, 20-μL AmpliScribe reaction will incorporate up to 90% of input NTPs and produce up to 150 μg of RNA from 1 μg of the control template. These yields are made possible by the high-performance properties of the AmpliScribe T7 enzyme preparations.

      AmpliScribe Kits produce exceptionally high yields of either long or short transcripts. The standard AmpliScribe reaction can be scaled up to produce milligram amounts of RNA. In addition, an AmpliScribe reaction can be readily modified to prepare fluorescent-¹, biotinylated-², or digoxigenin-³labeled RNA. Produce high yields of high quality RNA quickly and easily.

Formerly from Epicentre Synthesize long and short RNA with one kit

Produce >20 times more RNA than conventional or "homemade" in vitro transcription kits

Components:

AmpliScribe T7 Enzyme Solution, 

100 mM ATP, CTP, GTP, and UTP Solutions, 

AmpliScribe 10X Reaction Buffer, 

100 mM Dithiothreitol (DTT), 

RiboGuard? RNase Inhibitor, 

RNase-Free Water, 

Control Template DNA, and RNase-Free DNase I

參考文獻：

DeLong, E.F. et al., (1999) Appl. and Environ. Microbiol., 56, 5554.

Hoffman, L.M. and Johnson, M.G. (1994) BioTechniques 17, 372.

Kaplan, E.D. et al., (1996) Epicentre Forum, 3 (2), 1.

Molecular Cloning - A Laboratory Manual, Third Edition, 2001. CSHL Press. pp 7.27 –7.34. J. Sambrook and D. Russell.