當前位置： 首頁(yè) - 產(chǎn)品專(zhuān)區 - 熱銷(xiāo)產(chǎn)品
CopyCutter? Induction Solution/CopyCutter?誘導試劑
| 貨號 | CIS40025 | 售價(jià)（元） | 856 |
| 規格 | 25 mL | CAS號 |
- 產(chǎn)品簡(jiǎn)介
- 相關(guān)產(chǎn)品
產(chǎn)品信息
|
貨號 |
產(chǎn)品名稱(chēng) |
規格 |
價(jià)格/元 |
|
TNP92110 |
EZ-Tn5 Transposase/EZ-Tn5轉座酶 |
10 units |
6418 |
|
EZI982K |
EZ-Tn5? <KAN-2> Insertion Kit/EZ-Tn5? <KAN-2>插入試劑盒 |
10 rxns |
6950 |
|
EZI921T |
EZ-Tn5? <TET-1> Insertion Kit EZ-Tn5? <TET-1>插入試劑盒 |
10 rxns |
6999 |
|
EZI912D |
EZ-Tn5? <DHFR-1> Insertion Kit/EZ-Tn5? <DHFR-1>插入試劑盒 |
10 rxns |
7060 |
|
EZI03T7 |
EZ-Tn5? <T7/KAN-2> Promoter Insertion Kit/EZ-Tn5? <T7/KAN-2>啟動(dòng)子插入試劑盒 |
10 rxns |
7312 |
|
EZI011RK |
EZ-Tn5? <R6Kγori/KAN-2> Insertion Kit/EZ-Tn5? <R6Kγori/KAN-2>插入試劑盒 |
10 rxns |
7517 |
|
CIS40025 |
CopyCutter? Induction Solution/CopyCutter?誘導試劑 |
25 mL |
856 |
產(chǎn)品簡(jiǎn)介
CopyCutter? EPI400? Electrocompetent and Chemically Competent E. coli cells were developed to significantly lower the copy number of a wide variety of common vectors so that you can more readily clone unstable DNA sequences. Moreover, following a short incubation in the presence of the CopyCutter Induction Solution, you can subsequently raise copy number to improve plasmid yields without compromising stability.
CopyCutter?EPI400?電感受態(tài)和化學(xué)感受態(tài)大腸桿菌細胞的開(kāi)發(fā)可顯著(zhù)降低各種常見(jiàn)載體的拷貝數，因此您可以更輕松地克隆不穩定的DNA序列。此外，在存在CopyCutter誘導溶液的情況下進(jìn)行短暫孵育后，您可以隨后增加拷貝數以提高質(zhì)粒產(chǎn)量，而不會(huì )影響穩定性。
The CopyCutter EPI400 cell line was derived from our high-transformation efficiency phage T1-resistant TransforMax? EC100? E. coli strain by manipulating a gene that controls the copy number of vectors containing ColE1 or pMB1 origins of replication (e.g., pUC and pET type vectors). This constitutively expressed gene, pcnB (plasmid copy number), was deleted from the TransforMax EC100 strain and replaced with a modified pcnB gene linked to an inducible promoter, creating the CopyCutter EPI400 strain.
通過(guò)控制含有ColE1或pMB1復制起點(diǎn)（例如pUC和pET類(lèi)型）的載體的拷貝數的基因，CopyCutter EPI400細胞系源自我們的高轉化效率噬菌體T1抗性TransforMax?EC100?大腸桿菌菌株向量)。TransforMax EC100菌株中刪除了這個(gè)組成性表達的基因pcnB（質(zhì)?？截悢担?，并用與誘導型啟動(dòng)子連接的修飾的pcnB基因代替，從而創(chuàng )建了CopyCutter EPI400菌株。
優(yōu)點(diǎn)：
High transformation efficiency with clones of all sizes.
Supports blue/white screening of vectors.
Restriction minus [mcrA ?(mrr-hsdRMS-mcrBC)] for efficient cloning of methylated DNA.
Endonuclease minus (endA1) to ensure high yields of plasmid clones.
Recombination minus (recA1) to ensure the stability of large cloned inserts.
技術(shù)參數
產(chǎn)品優(yōu)點(diǎn)- High transformation efficiency with clones of all sizes.
- Supports blue/white screening of vectors.
- Restriction minus [mcrA ?(mrr-hsdRMS-mcrBC)] for efficient cloning of methylated DNA.
- Endonuclease minus (endA1) to ensure high yields of plasmid clones.
- Recombination minus (recA1) to ensure the stability of large cloned inserts.
產(chǎn)品應用- Stabilize toxic inserts in common cloning and expression vectors (pUC and pET-type vectors)
- Clone and maintain challenging sequences at reduced plasmid copy number, then induce to high copy number for DNA recovery
- Avoid T1 and T5 phage contamination with tonA mutation
- Choose chemically competent cells for general cloning or electrocompetent cells for demanding applications such as library generation