產(chǎn)品信息

      EpiScript? RNase H– Reverse Transcriptase (EpiScript RT), an alternative to SuperScript? II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity. It is  highly efficient at producing full-length cDNA from long RNA templates.  EpiScript RT demonstrates significantly more activity than other MMLV Reverse transcriptase enzymes. It is capable of producing cDNA from as little as 100pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.  
 EpiScript RT is supplied at a concentration of 200U/μL. A 10X RT Reaction Buffer and 100 mM dithiothreitol (DTT) are also provided.
 EpiScript?RNase H –逆轉錄酶（EpiScript RT）是SuperScript?II逆轉錄酶的替代品，是一種重組MMLV逆轉錄酶，其RNase H活性降低。 從長(cháng)RNA模板產(chǎn)生全長(cháng)cDNA的效率很高。EpiScript RT具有比其他MMLV逆轉錄酶更高的活性。它能夠從少至100pg的總RNA中產(chǎn)生cDNA，用于實(shí)時(shí)RT-PCR（qRT-PCR）分析和其他應用。
 EpiScript RT的濃度為200U /μL。還提供了10X RT反應緩沖液和100 mM二硫蘇糖醇（DTT）。
 EpiScript Reverse Trascriptase可以非常高效地從RNA模板產(chǎn)生長(cháng)度達12kb的全長(cháng)cDNA。該酶經(jīng)基因工程改造以顯著(zhù)降低RNase H活性。這種結構修飾消除了第一鏈cDNA合成過(guò)程中RNA分子的降解，使得EpiScrit Reverse Transcriptase在實(shí)時(shí)RT-PCR分析和其他應用中表現出卓越的性能。
優(yōu)點(diǎn)：
? Produce full-length cDNA with RNAse H- mutant；
? Achieve higher specificity at elevated temperatures, up to 55°C
組成成分：
? EpiScript RNase H- Reverse Transcriptase（200U/μL）：125μL； -20°C
? 10X Reaction Buffer：600μL；-20°C
? DTT（100 mM）：600μL；-20°C