產(chǎn)品信息

產(chǎn)品性質(zhì)

λabs 696 nm

λfl 718 nm

推薦STED損耗激光波長(cháng) 780-820 nm

lifetime 2.4 ns

MW 749.9

運輸 常溫

儲存 -20℃

能否用于固定細胞 支持任何方式固定的細胞

產(chǎn)品簡(jiǎn)介

SiR700-BG is a bright, cell permeable and far-red benzylguanine substrate for labeling SNAP-tag?* in cellular fluorescence imaging experiments.

Product contains 1 vial of SiR700-BG (35 nmol) in lyophilised form allowing to prepare 35 ul of 400x DMSO stock solution.

SiR700-BG is the benzylguanine derivative of our SiR700 fluorophore. It emits light in the far-red, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SiR700-BG can be imaged with a standard Cy5 filterset but using a 700 nm LP emission filter yields better results. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue.

Benzylguanine (BG) is the substrate of the self labeling tag SNAP-tag?*. Upon reaction with a BG derivative, SNAP-tag?* forms a covalent bond with the substrate and releases guanine. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as SNAP-tag?* fusion

*SNAP-tag? is a registered trademark of New England Biolabs, Inc.

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